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Neuroscience 2005 Abstract
| Presentation Number: |
143.13 |
| Abstract Title: |
Time course of neuronal marker expression during neurogenesis in the adult dentate gyrus of Rhesus monkey. |
| Authors: |
Kohler, S. J.*2;
Boklewski, J. L.4;
DeGrush, B. J.3;
Cameron, J. L.;
Greenough, W. T.1,2,3
1Psyc., Univ. of Illinois, Urbana-Champaign, Urbana, IL
2Neurosci., Univ. of Illinois, Urbana-Champaign, Urbana, IL
3CSB, Univ. of Illinois, Urbana-Champaign, Urbana, IL
4Bio, Univ. of Illinois, Urbana-Champaign, Urbana, IL
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| Primary Theme and Topics |
Development
- Neurogenesis and Gliogenesis
-- Proliferation
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| Session: |
143. Adult Mammalian Neurogenesis
Poster |
| Presentation Time: |
Sunday November 13, 2005 8:00 AM-9:00 AM |
| Location: |
Convention Center Exhibit Hall, Poster Board C17 |
| Keywords: |
Adult Neurogenesis, Primate, NeuN, BIII-tubulin
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The time course of neuronal marker expression in relation to adult hippocampal neurogenesis has not been studied in primate models. In rodents, different neuronal markers tend to be expressed at different time periods as adult generated hippocampal neurons mature. Our previous data (Kohler et al., 2004) suggests that the time course of expression of these markers in primates may be substantially different from that of rodents. This study investigates the course of expression of common markers of mature and immature neurons in adult dentate gyrus neurogenesis. 6 female Macaca mulatta monkeys were simultaneously given an i.p. injection of BrdU (100 mg/kg). Two animals were euthanized at each of three time points (48 h, 2 weeks and 6 weeks) after the injection. Neurogenesis was detected using antibodies directed against BrdU and the different neuronal markers. Confocal microscopy was used to verify co-labeling. At 48 hours all new cells were located in the subgraular zone. By week two most of the cells had moved into the granule cell layer. New cells expressing the immature neuronal marker ßIII- tubulin were not found at the 48 hour time point and its expression in new cells peaked at 2 weeks. This expression fells off at the 6 week time point. There were not a substantial number of new cells that stained with the mature neuronal marker NeuN by the six week time point.
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| Supported by Retirement Res Fndn; NIH/AG10154 |
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| Sample Citation: |
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[Authors]. [Abstract Title]. Program No. XXX.XX. 2005 Neuroscience Meeting Planner. Washington, DC: Society for Neuroscience, 2005. Online.
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| Copyright © 2005-2010 Society for Neuroscience; all rights reserved. Permission to republish any abstract or part of any abstract in any form must be obtained in writing by SfN office prior to publication.
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